Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers. for ValitaTiter Plus standards read on Molecular Devices readers. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. Flurescence intensities were read using a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. 0 OD for earlier models. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. These robust readers have been placed in labs from Antarctica to. 315 can't find zero order. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader Can you recommend disposable cuvettes for use in the microplate readers? Molecular Devices® does not recommend any specific disposable cuvette brands. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. Molecular Devices Spectramax M5 Microplate Reader. 7°C and the bottom to 36. Category 2 and 3 microplate readers use gradient heating as well. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck®. 00 USD. Choose cables that meet the requirement described in this appendix. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. However, when a specific probe is not available, or it causes off-target effects on the. The spectrophotometric. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Go to Product Details. 04 to 3. 3. Then, after H 2 O 2 was added, the fluorescence was measured for each time period at 488 nm (excitation) and 525 nm (emission) wavelengths on a SpectraMax M5 microplate reader. Microplate Readers. Conclusions We report a peptide-induced super-assembly of enzyme-containing MOFs for programmed enzyme. HTRF-compatible SpectraMax Multi-Mode Readers. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Wide range of applications. When a FlexStation® 3 Multi-Mode Microplate Reader. The following document is intended to demonstrate setup of this instrument. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. SDS-PAGE Assay. 2. SpectraMax® M5 Multi-Mode Microplate Reader (cat. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Depending on which. The calculated LOQ values were 0. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Shop now. Example: [Drawer]. We would like to show you a description here but the site won’t allow us. 0 out of 5 $ 16,499. When a feature or capability applies to only certain readers, this exception is noted. Ferguson, Ph. FlexStation™ III. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Table 1. Learn how you can utilize microplate readers to drive landmark discoveries. Our absorbance plate readers feature our PathCheck Sensor technology and. This. 2. 7 1. Step 2. Label: provides information about the reader, such as line voltage rating, caution-ary information, serial number, etc. 59 6. Use similar settings for other Molecular Devices readers with fluorescence detection mode. The absorbance was recorded using a SpectraMax M5 microplate reader (Molecular Devices). Microplate Readers Biotek Synergy H1 Hybrid Microplate. com. The cells were incubated with compounds at the indicated doses for 1 h. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Italic and boldface type are used for emphasis. #M5) SpectraMax® L Microplate Reader (cat. Page 28 Chapter 5: Read Modes and Read Types 1. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. es •. Keywords. both cuvette-based and microplate reader applications on the same instrument. Plate Shaking. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. All assays were performed in at least three individual experiments, each comprising no. TR-FRET assay. The readings with excitation at 540 nm and emission at 600 nm represented the amount of phosphorylated IκB-α in the cells. The samples were boiled for 5 min with 1 ×. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. ZERO BIAS - scores, article reviews, protocol conditions and moreThe absorbance was measured at 450–650 nm using a SpectraMAX M5 microplate reader (Molecular Devices). The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The drawing shows a standard microplate of 15 mm height covered with a lid. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. 2. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. SpectraMax® ABS Plus. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Compact and tunable absorbance microplate readers DATA SHEET. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Cuvette port functionality can also be controlled using SoftMax Pro Software. SpectraMax M Series Multi-Mode Microplate Readers 7 The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2µL samples with 24- or 64-well plates. 43 Max %CV 1. Data are normalized to vehicle-treated. Western blot analyses. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 1 second. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. SpectraMax 250 User. 00 USD. All associated StakMax® stackers. RAW 264. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Molecular Devices Spectramax M5 Microplate Reader. The CellTiter-Glo assay from Promega uses luciferase. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. "With this complete portfolio of multi-mode configurations, we. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. The guide also covers the software features, the specifications, and the accessories of the systems. ® reader to the computer. 14 Average %CV 0. Bookings are free, but you will need to use iLab to book. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Wide range of applications The VersaMax reader covers. • Tel. M3-M5e_manual. 2. 3 1. Molecular Devices. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. This assay is based on the disappearance of hydrogen peroxide (H 2 O 2) at 240 nm in a medium containing 20 mM H 2 O 2, 0. 1 & 7. free tracer decreases, resulting in. S. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. Microplate Readers are modular and . Microplate Readers include the M3, M4, M5, and M5e models. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. 2. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. . Next, add 10 μl of the MTT labeling reagent (5 mg/mL) to each well for 4 h. IMAP FP PDE Evaluation Kit (Molecular Devices P/N R8175)The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 0 out of. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. Page 28 Chapter 5: Read Modes and Read Types 1. book Page 7 Wednesday, July 7, 2010 2:09 PM. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. 2 only). Italic and boldface type are used for emphasis. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. Standard features include a. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. The chamber contains springs that position the cuvette in the proper alignment for a read. SpectraMax® M5e. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Fluorescence Microplate Readers. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuide SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. Make sure the microplate is flat against. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. SpectraMax® ABS Plus. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. book Page 7 Wednesday, July 7, 2010 2:09 PM. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Maximum signal with lowest background. Figure 4: Transcreener ADP. If you do not have an iLab account you must first make one: would like to show you a description here but the site won’t allow us. 05 3. 49 2. M5 SpectraMax M5e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5e models. 58% for the detection module and 2. The day before assay, assay buffer was prepared as described in the product technical manual. fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter. Add to Cart. played in a grid format that corresponds to the wells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Cell viability was quantified by measuring the absorbance of the solution at 570 nm using a SpectraMax® M5 microplate reader (Molecular Devices). The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . 1. Shimadzu 2450 UV–Vis spectrophotometer and SpectraMax M5 microplate reader were used to perform the optical characterization and measurements. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. 312 gain calibration failed. TR-FRET assay. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Figure 3. Physical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. upgradeable with a wide range of high performance capabilities ideal for life science research and drug. 6 for further experiment, so I'm confused which i would choose for OD value determination. l. Microplate Reader, MultimodeProducts : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. 6% for the on-board optics (monochromators). Table of Contents. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. You should get comparable results from top and bottom reads using EC50's to compare reads. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. Wash Buffer Concentrate was diluted 10-fold. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. 6-384. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. 313 reference gain check fail. Wide range of applications. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Microplate Capacity. 0 μL) instead of the enzyme solution was used as the sample blank group. Label-free detection. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. We use Spectramax M5 microplate reader in our lab. Design of genetic materials Sequence of β-gal siRNA was designed (5'-CA GUU GCG CAG CCU GAA UGU U3', 5'AA CAU UCA GGC UGC GCA ACU G-3') and nonsense siRNA ( Silencer ® negative control siRNA). The bound bio-peptidomimetics were recognized by Eu 3+-SA (diluted using 1% BSA in TBS), and the fluorescence intensities were measured using a SpectraMax M5 microplate reader after adding 200 μL signal enhancement solution, which the parameters were set as the followed: excitation and emission wavelengths of 340 and 615 nm,. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The readings with excitation at 360 nm and emission at 450 nm represented the amount of. 5 12. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Selection of Water Constant in PathCheck settings. IMAP FP Settings in SoftMax Pro M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Synergy H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. com A. No filter change with dual monochromator optics. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader;. SpectraMax® Aquamax. Molecular Devices. Temperature control range from Ambient +4º to 50ºC. Fluorescence from the Alamar blue® assay was detected by SpectraMax M5 microplate reader (Molecular Device Inc. , USA). 00 USD. SoftMax Pro. 4 Max StDev 1. When a feature or capability applies to only certain readers, this exception is noted. Fluorescence intensity was detected by. Comprehensive software and validation Comprehensive data analysis and GxP solutions SoftMax. SpectraMax® ABS. We specialize in the full suite of AKTA™ FPLC systems, Molecular Devices SpectraMax™ microplate readers, BioTek microplate washers, YMC (formerly LEWA) Process Technologies systems, ChromaCon Contichrom® instruments, and. Molecular Devices SpectraMax™ Plus 384 Microplate Reader (s) with One-Year Warranty $9,000. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. B: Selection of Excitation Wavelength. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Figure 1. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Choose from a three- (M3), four- (M4), or The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax M5 Multi-Mode Microplate Reader. The SpectraMax® M5 Multi-Mode Microplate Reader is ideally suited for assay development and high-throughput screening using IMAP assays. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Softmax Pro GxP Software 6. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Maximum signal with lowest background. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. 2. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. Add to Cart. When a feature or capability applies to only certain readers, this exception is noted. After two more rinses with Hank’s solution, fluorescence intensity of the red/green ratio was determined on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at an excitation of 490 nm and emission of 530 nm (green fluorescent monomers) and 590 nm (red fluorescent aggregates) respectively. SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Self Service Available. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. The following document is. Absorbance was read on a Spectramax M5 Microplate reader at 450 nm. Protocol Name Version 6. Designed to provide the simplicity, flexibility and power required for advanced data analysis. Figure 1. You can use either. FlexStation™ I. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Note: In this user guide, all references to SpectraMax Multi-Mode. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. Catalog Number: (89212-396) Supplier: Molecular Devices. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. The SpectraMax 190 reader can be integrated with Molecular . A control experiment of the activity of the proposed enzyme/ZIF-8A particles without peptides against protease digestion was also performed. e. The optical density (OD) was measured at 450 nm with a SpectraMax M5 microplate reader (Molecular Devices). 7 cells (6 × 10 5 cells/well in 6-well plates) were pretreated with of 3,3′,4,5′-TMS or 3,4′,5-TMS at 10 and 50 µM for 4 h, followed by the addition of LPS (1 µg/mL) for 12 h. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). 96-384. 1. When a. 95. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. TR-FRET assay principle. IMAP assays are based on binding of. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. No accessories are needed and SoftMax® Pro Software. The reader uses two scanning monochromators to determine the optimal excitation and emission settings for the spectral characteristics of. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. 1. KEY FEATURES • Five. 1. com. Fluorescence Microplate Readers. When wells are assigned to template groups, data will populate group tables where analysis canThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. SpectraMax iD3, iD5, i3x, i3, M3, M4, M5, M5e, SpectraMax L, 6186 Luminescence FlexStation 3. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. The drawing shows a standard microplate of 15 mm height covered with a lid. Urea-induced unfolding of rA1AT-WT and the Q9N mutant was measured using a SpectraMax. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates orThe optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. Learn how you can utilize microplate readers to drive landmark discoveries. Standard SDS-PAGE was performed with a 10% (w/v) separating gel and a 4% stacking gel. Microplate Readers are modular and . The cells were then treated to induce apoptosis. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Microplate Readers include the M3, M4, M5, and M5e models. Microplate Readers Include the M3, M4, M5, and M5. book Page 7 Wednesday, July 7, 2010 2:09 PM. Insert the cuvette into the chamber, making sure that the clear sides are to the left and right (facing the instrument). The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The cells were harvested on ice and. 40266 Email: drugdiscoverytech@lifetech. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. Fill a clean plate with water. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. Examples: “Press carefully to engage,” “ Do not. both cuvette-based and microplate reader applications The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Description. VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® Plus 384 Absorbance Microplate Reader; SpectraMax® L (software version 5. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. The advantage of using labeled probes is detection specificity. Bookings are free, but you will need to use iLab to book. Read More. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. Molecular Devices Spectramax M5 Microplate Reader; Related products. For readers without a cuvette port, these options are not shown in the software. 67. All reagents were brought to room temperature before use. Sensitive fluorescent quantitation of DNA with the Quant-iT. Figure 4: Transcreener ADP. Fresh cecum content samples were collected for 16S rRNA sequencing by Shanghai Majorbio Bio-Pharm Technology Co. The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. Sale price $18,499. Analyst, M5, Flex 3, and SpectraMax L readers work differently. These robust readers have been placed in labs from Antarctica to the.